NAME
ccs - Generate circular consensus sequences from subreads
SYNOPSIS
ccs [options] INPUT OUTPUT
DESCRIPTION
Generate circular consensus sequences (ccs) from subreads.
OPTIONS
- -h,--help
-
Output this help.
- --log-level,--logLevel
-
Set log level. ["INFO"]
- --version
-
Output version info.
- --force
-
Overwrite OUTPUT file if present.
- --zmws
-
Generate CCS for the provided comma-separated holenumber ranges only. Default = all
- --maxLength
-
Maximum length of subreads to use for generating CCS. [21000]
- --minLength
-
Minimum length of subreads to use for generating CCS. [10]
- --minPasses
-
Minimum number of subreads required to generate CCS. [3]
- --minPredictedAccuracy
-
Minimum predicted accuracy in [0, 1]. [0.9]
- --minIdentity
-
Minimum identity to the POA to use a subread. 0 disables this filter. [0.82]
- --minZScore
-
Minimum z-score to use a subread. NaN disables this filter. [-3.4]
- --maxDropFraction
-
Maximum fraction of subreads that can be dropped before giving up. [0.34]
- --minSnr
-
Minimum SNR of input subreads. [3.75]
- --minReadScore
-
Minimum read score of input subreads. [0.75]
- --byStrand
-
Generate a consensus for each strand.
- --noPolish
-
Only output the initial template derived from the POA (faster, less accurate).
- --polish
-
Emit high-accuracy CCS sequences polished using the Arrow algorithm
- --polishRepeats
-
Polish repeats of 2 to N bases of 3 or more elements. [0]
- --richQVs
-
Emit dq, iq, and sq "rich" quality tracks.
- --reportFile
-
Where to write the results report. ["ccs_report.txt"]
- --modelPath
-
Path to a model file or directory containing model files.
- --modelSpec
-
Name of chemistry or model to use, overriding default selection.
- --numThreads
-
Number of threads to use, 0 means autodetection. [0]
- --logFile
-
Log to a file, instead of STDERR.
- --emit-tool-contract
-
Emit tool contract.
- --resolved-tool-contract
-
Use args from resolved tool contract.
Arguments:
- input
-
Input file.
- output
-
Output file.
AUTHOR
This manpage was written by Andreas Tille for the Debian distribution and can be used for any other usage of the program.