---
name: "twopaco"
section: "1"
platform: "linux"
package: "twopaco"
version: "1.0.0+dfsg-1"
release: "Debian 13 (Trixie)"
platforms: ["linux"]
url: "https://new.carta.tech/twopaco"
---

# twopaco(1)

build the compacted de Bruijn graph from many complete genomes

## NAME

twopaco - build the compacted de Bruijn graph from many complete genomes

## DESCRIPTION

USAGE:

- twopaco

  {-f \<integer\>\|--filtermemory \<float\>} \[-o \<file name\>\] \[--test\] \[--tmpdir \<directory name\>\] \[-a \<integer\>\] \[-t \<integer\>\] \[-r \<integer\>\] \[-q \<integer\>\] \[-k \<oddc\>\] \[--\] \[--version\] \[-h\] \<fasta files with genomes\> ...

Where:

**-f** \<integer\>,  **--filtersize** \<integer\>

- (OR required)

  Size of the filter

**--** OR **--**

**--filtermemory** \<float\>

- (OR required)

  Memory in GBs allocated for the filter

**-o** \<file name\>,  **--outfile** \<file name\>

-

  Output file name prefix

**--test**

-

  Run tests

**--tmpdir** \<directory name\>

-

  Temporary directory name

**-a** \<integer\>,  **--abundance** \<integer\>

-

  Vertex abundance threshold

**-t** \<integer\>,  **--threads** \<integer\>

-

  Number of worker threads

**-r** \<integer\>,  **--rounds** \<integer\>

-

  Number of computation rounds

**-q** \<integer\>,  **--hashfnumber** \<integer\>

-

  Number of hash functions

**-k** \<oddc\>,  **--kvalue** \<oddc\>

-

  Value of k

**--**,  **--ignore\_rest**

-

  Ignores the rest of the labeled arguments following this flag.

**--version**

-

  Displays version information and exits.

**-h**,  **--help**

-

  Displays usage information and exits.

- \<fasta files with genomes\>

  (accepted multiple times)

- (required)

  FASTA file(s) with nucleotide sequences.

-

  Program for construction of the condensed de Bruijn graph from complete genomes

## AUTHOR

 This manpage was written by Andrius Merkys for the Debian distribution and\
  can be used for any other usage of the program.