---
name: "tradis_essentiality"
section: "1"
platform: "linux"
package: "bio-tradis"
version: "1.4.5+dfsg2-2"
release: "Debian 13 (Trixie)"
platforms: ["linux"]
url: "https://new.carta.tech/tradis_essentiality"
---

# tradis\_essentiality(1)

bio-tradis: produces calls of gene essentiality using an adaptation of the method described in Langridge et al.

## NAME

tradis\_essentiality - bio-tradis: produces calls of gene essentiality using an adaptation of the method described in Langridge et al.

## SYNOPSIS

**tradis\_essentiality** *data.tab*

## DESCRIPTION

Produces calls of gene essentiality using an adaptation of the method described in Langridge et al. Genome Research 2009 and Barquist et al. NAR 2013. A loess curve is fit to the distribution of insertion indices, and used to identify the minima between the 'essential' and 'non-essential' distributions. These distributions are then used to fit gamma distributions, which are then used to calculate log-odds ratios, which are used to determine an insertion-index threshold for gene essentiality. Note that this analysis requires a saturated mutant library, and is not suitable for the analysis of data sets with low insertion density. The script produces a number of diagnostic plots which can be used to verify that this condition has been met.

## AUTHOR

This manpage was written by Andreas Tille for the Debian distribution and can be used for any other usage of the program.