---
name: "rsem-control-fdr"
section: "1"
platform: "linux"
package: "rsem"
version: "1.3.3+dfsg-3+b1"
release: "Debian 13 (Trixie)"
platforms: ["linux"]
url: "https://new.carta.tech/rsem-control-fdr"
---

# rsem-control-fdr(1)

Filter EBSeq output for statistical significance.

## NAME

rsem-control-fdr - Filter EBSeq output for statistical significance.

## SYNOPSIS

rsem-control-fdr \[options\] input\_file fdr\_rate output\_file

## ARGUMENTS

- **input\_file**

  This should be the main result file generated by 'rsem-run-ebseq', which contains all genes/transcripts and their associated statistics.

- **fdr\_rate**

  The desire false discovery rate (FDR).

- **output\_file**

  This file is a subset of the 'input\_file'. It only contains the genes/transcripts called as differentially expressed (DE). When more than 2 conditions exist, DE is defined as not all conditions are equally expressed.  Because statistical significance does not necessarily mean biological significance, users should also refer to the fold changes to decide which genes/transcripts are biologically significant. When more than two conditions exist, this file will not contain fold change information and users need to calculate it from 'input\_file.condmeans' by themselves.

## OPTIONS

- **--hard-threshold**

  Use hard threshold method to control FDR. If this option is set, only those genes/transcripts with their PPDE \>= 1 - fdr\_rate are called as DE. (Default: on)

- **--soft-threshold**

  Use soft threshold method to control FDR. If this option is set, this program will try to report as many genes/transcripts as possible, as long as their average PPDE \>= 1 - fdr\_rate. This option is equivalent to use EBSeq's 'crit\_fun' for FDR control. (Default: off)

- **-h/--help**

  Show help information.

## DESCRIPTION

This program controls the false discovery rate and reports differentially expressed genes/transcripts.

## EXAMPLES

We assume that we have 'GeneMat.results' as input. We want to control FDR at 0.05 using hard threshold method and name the output file as 'GeneMat.de.txt':

```
 rsem-control-fdr GeneMat.results 0.05 GeneMat.de.txt
```