---
name: "prima"
section: "1e"
platform: "linux"
package: "emboss"
version: "6.6.0+dfsg-15"
release: "Debian 13 (Trixie)"
platforms: ["linux"]
url: "https://new.carta.tech/prima"
---

# prima(1e)

Selects primers for PCR and DNA amplification.

## NAME

prima - Selects primers for PCR and DNA amplification.

## SYNOPSIS

**prima** **-sequence** *sequence* **-targetrange** *toggle* **-targetstart** *integer* **-targetend** *integer* **-mintmprimer** *float* **-maxtmprimer** *float* **-minplen** *integer* **-maxplen** *integer* **-minpgccont** *float* **-maxpgccont** *float* \[**-primer** *toggle*\] **-minprimerlen** *integer* **-maxprimerlen** *integer* **-minpmgccont** *float* **-maxpmgccont** *float* **-overlap** *integer* **-saltconc** *float* **-dnaconc** *float* **-list** *boolean* **-outfile** *outfile*

**prima** **-help**

## DESCRIPTION

**prima** is a command line program from EMBOSS (“the European Molecular Biology Open Software Suite”). It is part of the "Nucleic:Primers" command group(s).

## OPTIONS

### Input section

- **-sequence** *sequence*

  ### Required section

### Target options

- **-targetrange** *toggle*

  Default value: N

- **-targetstart** *integer*

  Default value: $(sequence.begin)

- **-targetend** *integer*

  Default value: $(sequence.length)

- **-mintmprimer** *float*

  Default value: 53

- **-maxtmprimer** *float*

  Default value: 58

### Additional section

### Product options

- **-minplen** *integer*

  Default value: 100

- **-maxplen** *integer*

  Default value: 300

- **-minpgccont** *float*

  Default value: .40

- **-maxpgccont** *float*

  Default value: .55

### Primer options

- **-primer** *toggle*

  Specify primer properties Default value: Y

- **-minprimerlen** *integer*

  Default value: 18

- **-maxprimerlen** *integer*

  Default value: 22

- **-minpmgccont** *float*

  Default value: .40

- **-maxpmgccont** *float*

  Default value: .55

### Advanced section

- **-overlap** *integer*

  Default value: 50

- **-saltconc** *float*

  Default value: 50

- **-dnaconc** *float*

  Default value: 50

- **-list** *boolean*

  Default value: N

### Output section

- **-outfile** *outfile*

  ## BUGS

Bugs can be reported to the Debian Bug Tracking system (http://bugs.debian.org/emboss), or directly to the EMBOSS developers (http://sourceforge.net/tracker/?group\_id=93650&atid=605031).

## SEE ALSO

prima is fully documented via the **tfm**(1) system.

## AUTHOR

**Debian Med Packaging Team** \<debian-med-packaging@lists.alioth.debian.org\>

Wrote the script used to autogenerate this manual page.

## COPYRIGHT

This manual page was autogenerated from an Ajax Control Definition of the EMBOSS package. It can be redistributed under the same terms as EMBOSS itself.