proftmb1
Per-residue prediction of bacterial transmembrane beta barrels
Usage
proftmb [options]
Overview
proftmb predicts bacterial transmembrane beta barrels from sequence using profile fed Hidden Markov Models (HMM).
Output format
Self-annotating. Letters of prediction: 'i' - inside cytoplasm, 'O' - outside cytoplasm, 'U' - up (on chain moving out of cytoplasm), 'D' - down (on chain moving into cytoplasm).
References
- Bigelow, H. and Rost, B. (2006). PROFtmb: a web server for predicting bacterial transmembrane beta barrel proteins. Nucleic Acids Res, 34(Web Server issue), W186-8.
To invoke the menu, type:
-
proftmb
--menu
The options are:
-
-d, --directory-root <STRING> -
root path where files (options
-s,-r,-l,-a,-e,-t,-u,-z,-n) reside
-
-a, --reduction-state-decode <STRING> -
state reduction for decoding
-
-b, --reduction-state-report <STRING> -
state reduction for reporting
-
-m, --membrane-strand-states <STRING> -
list of membrane strand states
-
-z, --z-curve-file -
<STRING> file containing means and sd's at integral length values
-
-x, --z-calibration-curve <STRING> -
file mapping coverage and accuracy values to z-scores
-
-n, --null-frequency <STRING> -
background frequency file
-
-c, --minimum-score-cutoff <DOUBLE> -
minimum z-score for per-residue prediction
-
-o, --outfile-prefix <STRING> -
output file prefix for the three files generated: PREFIX_dat.txt, PREFIX_proftmb_pretty.txt PREFIX_proftmb_tabular.txt
-
-s, --static-model-data <STRING> -
data representing the model architecture
-
-t, --trained-params <STRING> -
params representing the encoded training data
-
-q, --test-blastQ-file-or-dir <STRING> -
psiblast profile (
-Q) or directory (full pathname or relative to current directory) with many profiles
-
-w, --single-sequence-name <STRING> -
if
-qoption points to a single file, this is the name
-
-v, --list-blastQ-files <STRING> -
list of psiblast files to process in directory (leave blank to process all files)
-
--outfile-pretty <STRING> -
pretty output file (overrides automatic name PREFIX_proftmb_pretty.txt)
-
--outfile-tab -
<STRING> tabulated output file (overrides automatic name PREFIX_proftmb_tabular.txt)
-
--outfile-dat -
<STRING> data output file (overrides automatic name PREFIX_dat.txt)
-
--version -
<BOOL> output version information and exit
-
--quiet -
<BOOL> be quiet
Examples
proftmb @/usr/share/proftmb/options -q /usr/share/doc/proftmb/examples/example.Q -o /tmp/example
The above produces 3 output files: /tmp/example_proftmb_tabular.txt, /tmp/example_proftmb_pretty.txt and /tmp/example_dat.txt. Compare these to the examples in /usr/share/doc/proftmb/examples.
Releases
7 releases since Debian 7 (Wheezy); the text changed 6 times.