bp_process_wormbase1p

Usage

  % bp_process_wormbase.pl ./WS61 > wormbase.gff

Overview

This script massages the Wormbase GFF files located at ftp://www.wormbase.org/pub/wormbase/GENE_DUMPS into a version of the GFF format suitable for display by the generic genome browser. It mainly adds comments to the annotations and designates certain well-spaced genetic loci as framework landmarks.

This script requires the AcePerl distribution, which is available on CPAN (look for the "Ace" module).

To use this script, get the WormBase GFF files from the FTP site listed above and place them in a directory. It might be a good idea to name the directory after the current release, such as WS61. You do not need to uncompress the files.

Then give that directory as the argument to this script and capture the script's output to a file:

  % bp_process_wormbase.pl ./WS61 > wormbase.gff

It may take a while before you see output from this script, since it must first fetch gene and protein database from the remote AceDB running at www.wormbase.org. The wormbase.gff file can then be loaded into a Bio::DB::GFF database using the following command:

  % bulk_load_gff.pl -d <databasename> wormbase.gff