carta
idba_hybrid(1)

idba_hybrid1

Iterative de bruijn graph assembler for hybrid sequencing data.

Usage

idba_hybrid -r read.fa -o output_dir [--reference ref.fa]

Overview

IDBA-Hybrid - Iterative de Bruijn Graph Assembler for hybrid sequencing data.

Allowed Options:

-o, --out arg (=out)

output directory

-r, --read arg

fasta read file (<=128)

--read_level_2 arg

paired-end reads fasta for second level scaffolds

--read_level_3 arg

paired-end reads fasta for third level scaffolds

--read_level_4 arg

paired-end reads fasta for fourth level scaffolds

--read_level_5 arg

paired-end reads fasta for fifth level scaffolds

-l, --long_read arg

fasta long read file (>128)

--reference arg

reference genome

--mink arg (=20)

minimum k value (<=124)

--maxk arg (=100)

maximum k value (<=124)

--step arg (=20)

increment of k-mer of each iteration

--inner_mink arg (=10)

inner minimum k value

--inner_step arg (=5)

inner increment of k-mer

--prefix arg (=3)

prefix length used to build sub k-mer table

--min_count arg (=2)

minimum multiplicity for filtering k-mer when building the graph

--min_support arg (=1)

minimum supoort in each iteration

--num_threads arg (=0)

number of threads

--seed_kmer arg (=30)

seed kmer size for alignment

--min_contig arg (=200)

minimum size of contig

--min_region arg (=500)

minimum size of region in reference genome

--similar arg (=0.95)

similarity for alignment

--max_mismatch arg (=3)

max mismatch of error correction

--min_pairs arg (=3)

minimum number of pairs

--max_gap arg (=50)

maximum gap in reference

--no_local

do not use local assembly

--no_coverage

do not iterate on coverage

--no_correct

do not do correction

--pre_correction

perform pre-correction before assembly

History

  • Debian 13 (Trixie) to Debian 11 (Bullseye): the text shown here
  • Debian 10 (Buster)
  • Debian 9 (Stretch) to Debian 8 (Jessie)

Author

This manpage was written by Andreas Tille for the Debian distribution and
can be used for any other usage of the program.

From idba 1.1.3-8 in Debian 13 (Trixie). Parsed by carta from the page's man source.