NAME
TFBS::DB::JASPAR4 - interface to MySQL relational database of pattern matrices
SYNOPSIS
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creating a database object by connecting to the existing JASPAR2-type database
my $db = TFBS::DB::JASPAR4->connect("dbi:mysql:JASPAR4:myhost","myusername","mypassword");
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retrieving a TFBS::Matrix::* object from the database
# retrieving a PFM by IDmy $pfm = $db->get_Matrix_by_ID('M0079','PFM');#retrieving a PWM by namemy $pwm = $db->get_Matrix_by_name('NF-kappaB', 'PWM');
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retrieving a set of matrices as a TFBS::MatrixSet object according to various criteria
# retrieving a set of PWMs from a list of IDs:my @IDlist = ('M0019', 'M0045', 'M0073', 'M0101');my $matrixset = $db->get_MatrixSet(-IDs => \@IDlist,-matrixtype => "PWM");# retrieving a set of ICMs from a list of names:my @namelist = ('p50', 'p53', 'HNF-1'. 'GATA-1', 'GATA-2', 'GATA-3');my $matrixset = $db->get_MatrixSet(-names => \@namelist,-matrixtype => "ICM");# retrieving a set of all PFMs in the database# derived from human genes:my $matrixset = $db->get_MatrixSet(-species => ['Homo sapiens'],-matrixtype => "PFM");
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creating a new JASPAR4-type database named MYJASPAR4:
my $db = TFBS::DB::JASPAR4->create("dbi:mysql:MYJASPAR4:myhost","myusername","mypassword");
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storing a matrix in the database (currently only PFMs):
#let $pfm is a TFBS::Matrix::PFM object$db->store_Matrix($pfm);
DESCRIPTION
TFBS::DB::JASPAR4 is a read/write database interface module that retrieves and stores TFBS::Matrix::* and TFBS::MatrixSet objects in a relational database. The interface is nearly identical to the JASPAR2interface, while the underlying data model is different
JASPAR2 DATA MODEL
JASPAR4 is working name for a relational database model used for storing transcriptional factor pattern matrices in a MySQL database. It was initially designed (JASPAR2) to store matrices for the JASPAR database of high quality eukaryotic transcription factor specificity profiles by Albin Sandelin and Wyeth W. Wasserman. Besides the profile matrix itself, this data model stores profile ID (unique), name, structural class, basic taxonomic and bibliographic information as well as some additional opseqdbtional tags.
Tags that are commonly used in the actual JASPAR database include
'medline' # PubMed ID
'species' # Species name
'superclass' #Species supergroup, eg 'vertebrate', 'plant' etc
'total_ic' # total information content - redundant, present
# for historical
'type' #experimental nethod
'acc' #accession number for TF protein sequence
'seqdb' #corresponding database name
but any tag is storable and searchable.
----------------------- ADVANCED ---------------------------------
For the developers and the curious, here is the JASPAR4 data model:
It is our best intention to hide the details of this data model, which we are using on a daily basis in our work, from most TFBS users. Most users should only know the methods to store the data and which tags are supported.
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FEEDBACK
Please send bug reports and other comments to the author.
AUTHOR - Boris Lenhard
Boris Lenhard <[email protected]>
APPENDIX
The rest of the documentation details each of the object methods. Internal methods are preceded with an underscore.
new
Title : newUsage : DEPRECATED - for backward compatibility onlyUse connect() or create() instead
connect
Title : connectUsage : my $db =TFBS::DB::JASPAR4->connect("dbi:mysql:DATABASENAME:HOSTNAME","USERNAME","PASSWORD");Function: connects to the existing JASPAR4-type database andreturns a database object that interfaces the databaseReturns : a TFBS::DB::JASPAR4 objectArgs : a standard database connection triplet("dbi:mysql:DATABASENAME:HOSTNAME", "USERNAME", "PASSWORD")In place of DATABASENAME, HOSTNAME, USERNAME and PASSWORD,use the actual values. PASSWORD and USERNAME might beoptional, depending on the user's access permissions forthe database server.
dbh
Title : dbhUsage : my $dbh = $db->dbh();$dbh->do("UPDATE matrix_data SET name='ADD1' WHERE NAME='SREBP2'");Function: returns the DBI database handle of the MySQL databaseinterfaced by $db; THIS IS USED FOR WRITING NEW METHODSFOR DIRECT RELATIONAL DATABASE MANIPULATION - if youhave write access AND do not know what you are doing,you can severely corrupt the dataFor documentation about database handle methods, see L<DBI>Returns : the database (DBI) handle of the MySQL JASPAR2-typerelational database associated with the TFBS::DB::JASPAR2objectArgs : none
store_Matrix
Title : store_MatrixUsage : $db->store_Matrix($matrixobject);Function: Stores the contents of a TFBS::Matrix::DB object in the databaseReturns : 0 on success; $@ contents on failure(this is too C-like and may change in future versions)Args : (PFM_object)A TFBS::Matrix::PFM, FBS::Matrix::PWM or FBS::Matrix::ICM object.PFM object are recommended to use, as they are eaily converted toother formatsComment : this is an experimental method that is not 100% bulletproof;use at your own risk
get_Matrix_by_ID
Title : get_Matrix_by_IDUsage : my $pfm = $db->get_Matrix_by_ID('M00034', 'PFM');Function: fetches matrix data under the given ID from thedatabase and returns a TFBS::Matrix::* objectReturns : a TFBS::Matrix::* object; the exact type of theobject depending on what form the matrix is storedin the database (PFM is default)Args : (Matrix_ID)Matrix_ID is a string;
get_Matrix_by_name
Title : get_Matrix_by_nameUsage : my $pfm = $db->get_Matrix_by_name('HNF-1');Function: fetches matrix data under the given name from thedatabase and returns a TFBS::Matrix::* objectReturns : a TFBS::Matrix::* object; the exact type of the objectdepending on what form the matrix object was stored inthe database (default PFM))Args : (Matrix_name)Warning : According to the current JASPAR4 data model, name isnot necessarily a unique identifier. In the case wherethere are several matrices with the same name in thedatabase, the function fetches the first one and printsa warning on STDERR. You've been warned.
get_MatrixSet
Title : get_MatrixSetUsage : my $matrixset = $db->get_MatrixSet(%args);Function: fetches matrix data under for all matrices in the databasematching criteria defined by the named argumentsand returns a TFBS::MatrixSet objectReturns : a TFBS::MatrixSet objectArgs : This method accepts named arguments, corresponding to arbitrary tags.Note that this is different from JASPAR2. As any tag is supported fordatabase storage, any tag can be used for information retrieval.Additionally, arguments as 'name' and 'class' can be used (even thoughthey are not tags.As with get_Matrix methods, it is important to realize that any matrixformat can be stored in the database: the TFBS::MatrixSet might thereforeconsist of PFMs, ICMs and PWMS, depending on how matrices are stored,Examples include-ID # a reference to an array of IDs (strings)-name # a reference to an array of# transcription factor names (string)-class # a reference to an array of# structural class names (strings)-species # a reference to an array of# Latin species names (strings)-sysgroup # a reference to an array of# higher taxonomic categories (strings)-min_ic # float, minimum total information content# of the matrix. IMPORTANT:if retrieved matrices are in PWMformat there is no way to measureinformation content.-matrixtype #string describing type of matrix to retrieve. If left out, the formatwill revert to the database format. Note that this option only worksif the database format is pfm
The arguments that expect list references are used in database query formulation: elements within lists are combined with 'OR' operators, and the lists of different types with 'AND'. For example,
my $matrixset = $db->(-class => ['TRP_CLUSTER', 'FORKHEAD'],-species => ['Homo sapiens', 'Mus musculus'],);
gives a set of TFBS::Matrix::PFM objects (given that the matrix models are stored as such)
whose (structural clas is 'TRP_CLUSTER' OR'FORKHEAD') AND (the species they are derived
from is 'Homo sapiens'OR 'Mus musculus').
The -min_ic filter is applied after the query in the sense that the matrices profiles with total information content less than specified are not included in the set.
delete_Matrix_having_ID
Title : delete_Matrix_having_IDUsage : $db->delete_Matrix_with_ID('M00045');Function: Deletes the matrix having the given ID from the databaseReturns : 0 on success; $@ contents on failure(this is too C-like and may change in future versions)Args : (ID)A stringComment : Yeah, yeah, 'delete_Matrix_having_ID' is a stupid namefor a method, but at least it should be obviuos what it does.