---
name: "FastaUnique"
section: "1"
platform: "linux"
package: "ampliconnoise"
version: "1.29-15+b1"
release: "Debian 13 (Trixie)"
platforms: ["linux"]
url: "https://new.carta.tech/ampliconnoise"
---

# FastaUnique(1)

remove noise from high throughput nucleotide sequence data

Part of [ampliconnoise(1)](/ampliconnoise/1.md).

## NAME

AmpliconNoise - remove noise from high throughput nucleotide sequence data

## VERSION

This documentation refers to version 1.22

## SYNOPSIS

See **/usr/share/doc/ampliconnoise/Doc.pdf.gz** for details of how to run.

## DESCRIPTION

The following tools are included.  Most of them have an MPI equivalent, for example SeqNoise has an equivalent SeqNoiseM which can be used with mpirun.

**FastaUnique** - dereplicates fasta file\
   \-in     string            input file name\
  Options:

**FCluster**\
   \-in    string            distance input file name\
   \-out   string            output file stub\
  Options:\
   \-r                       resolution\
   \-a                       average linkage\
   \-w                       use weights\
   \-i                       read identifiers\
   \-s                       scale dist.

**NDist** - pairwise Needleman-Wunsch sequence distance matrix from a fasta file\
   \-in     string            fata file name\
  Options:\
   \-i output identifiers

**Perseus** - slays monsters\
   \-sin     string            seq file name\
  Options:\
   \-tin     string            reference sequence file\
   \-a                         output alignments\
   \-d                         use imbalance\
   \-rin     string            lookup file name

**PyroDist** - pairwise distance matrix from flowgrams\
   \-in     string            flow file name\
   \-out    stub              out file stub\
  Options:\
   \-ni                       no index in dat file\
   \-rin    string            lookup file name

**PyroNoise** - clusters flowgrams without alignments\
   \-din     string            flow file name\
   \-out     string            cluster input file name\
   \-lin     string            list file\
  Options:\
   \-v       verbose\
   \-c       double            initial cut-off\
   \-ni                        no index in dat file\
   \-s       double            precision\
   \-rin     file              lookup file name

**SeqDist** - pairwise distance matrix from a fasta file\
   \-in     string            fasta file name\
  Options:\
   \-i output identifiers\
   \-rin    string            lookup file name

**SeqNoise** - clusters sequences\
   \-in      string            sequence file name\
   \-din     string            distance matrix file name\
   \-out     string            cluster input file name\
   \-lin     string            list file\
  Options:\
   \-min       mapping file\
   \-v       verbose\
   \-c       double            initial cut-off\
   \-s       double            precision\
   \-rin     string            lookup file name

**SplitClusterEven**\
   \-din    string  dat filename\
   \-min    string  map filename\
   \-tin    string  tree filename\
   \-s      split size\
   \-m      min   size

## AUTHOR

All software by Chris Quince (quince@civil.gla.ac.uk) This manpage by Tim Booth  (tbooth@ceh.ac.uk)

## LICENCE AND COPYRIGHT

Copyright (c) 2009  (quince@civil.gla.ac.uk). All rights reserved.

Released under the Lesser GPL.

Permission is granted for anyone to copy, use, or modify these programs and documents  for purposes of research or education, provided this copyright notice is retained,  and note is made of any changes that have been made.