Reference

Bio::SeqIO::game::gameWriter(3pm)

NAME

Bio::SeqIO::game::gameWriter -- a class for writing game-XML

SYNOPSIS

  use Bio::SeqIO;
  my $in  = Bio::SeqIO->new( -format => 'genbank',
                             -file => 'myfile.gbk' );
  my $out = Bio::SeqIO->new( -format => 'game',
                             -file => 'myfile.xml' );
  # get a sequence object
  my $seq = $in->next_seq;
  #write it in GAME format
  $out->write_seq($seq);

DESCRIPTION

Bio::SeqIO::game::gameWriter writes GAME-XML (v. 1.2) that is readable by Apollo. It is best not used directly. It is accessed via Bio::SeqIO.

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists.

Your participation is much appreciated.

  [email protected]                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Support

Please direct usage questions or support issues to the mailing list:

[email protected]

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:

  https://github.com/bioperl/bioperl-live/issues

AUTHOR - Sheldon McKay

Email [email protected]

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

new

 Title   : new
 Usage   : my $writer = Bio::SeqIO::game::gameWriter->new($seq);
 Function: constructor method for gameWriter 
 Returns : a game writer object 
 Args    : a Bio::SeqI implementing object
           optionally, an argument to set map_position to on.
           ( map => 1 ).  This will create a map_position elemant
           that will cause the feature coordinates to be remapped to
           a parent seqeunce.  A sequence name in the format seq:xxx-xxx
           is expected to determine the offset for the map_position.
           The default behavior is to have features mapped relative to 
           the sequence contained in the GAME-XML file

write_to_game

 Title   : write_to_game
 Usage   : $writer->write_to_game
 Function: writes the sequence object to game-XML 
 Returns : xml as a multiline string
 Args    : none

_rearrange_hierarchies

 Title   : _rearrange_hierarchies
 Usage   : $self->_rearrange_hierarchies($seq)
 Function: internal method to rearrange gene containment hierarchies
           so that snRNA or transposon features contain their genes
           rather than the other way around
 Returns : nothing
 Args    : a Bio::RichSeq object
 Note    : Not currently used, may be removed

_write_feature

 Title   : _write_feature
 Usage   : $seld->_write_feature($feat, 1)
 Function: internal method for writing generic features as <annotation> elements
 Returns : nothing
 Args    : a Bio::SeqFeature::Generic object and an optional flag to write a
           bare feature set with no annotation wrapper

_write_gene

 Title   : _write_gene
 Usage   : $self->_write_gene($feature)
 Function: internal method for rendering gene containment hierarchies into 
           a nested <annotation> element 
 Returns : nothing
 Args    : a nested Bio::SeqFeature::Generic gene feature
 Note    : A nested gene hierarchy (gene->mRNA->CDS->exon) is expected.  If other gene 
           subfeatures occur as level one subfeatures (same level as mRNA subfeats) 
           an attempt will be made to link them to transcripts via the 'standard_name'
           qualifier

_check_cds

 Title   : _check_cds
 Usage   : $self->_check_cds($cds, $name)
 Function: internal method to check if the CDS associated with an mRNA is
           the correct alternative splice variant
 Returns : a Bio::SeqFeature::Generic CDS object
 Args    : the CDS object plus the transcript\'s 'standard_name'
 Note    : this method only works if alternatively spliced transcripts are bound
           together by a 'standard_name' or 'mRNA' qualifier.  If none is present, 
           we will hope that the exons were derived from a segmented RNA or a CDS 
           with no associated mRNA feature.  Neither of these two cases would be 
           confounded by alternative splice variants.

_comp_analysis

  Usage:
  Desc :
  Ret  :
  Args :
  Side Effects:
  Example:

_comp_result

  Usage:
  Desc : recursively render a feature and its subfeatures as
         <result_set> and <result_span> elements
  Ret  : nothing meaningful
  Args : a feature

_comp_result_span

  Usage: _comp_result_span('foo12',$feature);
  Desc : write GAME XML for a Bio::SeqFeature::Computation feature
         that has no subfeatures
  Ret  : nothing meaningful
  Args : name for this span (some kind of identifier),
         SeqFeature object to put into this span
  Side Effects:
  Example:

_render_tags

  Usage:
  Desc :
  Ret  :
  Args :
  Side Effects:
  Example:

_render_output_tags

  Usage:
  Desc : print out <output> elements, with contents
         taken from the SeqFeature::Computation's 'output' tag
  Ret  : array of tag names this did not render
  Args : feature object, list of tag names to maybe render
  In game xml, only <result_span> and <result_set> elements can
  have <output> elements.

_render_tags_as_properties

  Usage:
  Desc :
  Ret  : empty array
  Args : feature object, array of tag names
  Side Effects:
  Example:
  In game xml, <annotation>, <computational_analysis>,
  and <feature_set> elements can have properties.

_render_comment_tags

  Usage:
  Desc :
  Ret  : names of tags that were not comment tags
  Args : feature object, tag names available for us to render
  Side Effects: writes XML
  Example:
  In game xml, <annotation> and <feature_set> elements can
  have comments.

_render_date_tags

  Usage:
  Desc :
  Ret  : names of tags that were not date tags
  Args : feature, list of tag names available for us to render
  Side Effects: writes XML for <date> elements
  Example:
  In game xml, <annotation>, <computational_analysis>,
  <transaction>, <comment>, and <feature_set> elements
  can have <date>s.

_render_dbxref_tags

  Desc : look for xref tags and render them if they are there
  Ret  : tag names that we didn't render
  Args : feature object, list of tag names to render
  Side Effects: writes a <dbxref> element if a tag with name
                matching /xref$/i is present
  In game xml, <annotation> and <seq> elements can have dbxrefs.

_render_target_tags

  Usage:
  Desc : process any 'Target' tags that would indicate a sequence alignment subject
  Ret  : array of tag names that we didn't render
  Args : feature object
  Side Effects: writes a <seq_relationship> of type 'subject' if it finds
                any properly formed tags named 'Target'
  Example:
  In game xml, <result_span>, <feature_span>, and <result_set> can have
  <seq_relationship>s.  <result_set> can only have one, a 'query' relation.

_property

 Title   : _property
 Usage   : $self->_property($tag => $value); 
 Function: an internal method to write property XML elements
 Returns : nothing
 Args    : a tag/value pair

_unflatten_attribute

 Title   : _unflatten_attribute
 Usage   : $self->_unflatten_attribute($name, $value)
 Function: an internal method to unflatten and write comment or evidence elements
 Returns : nothing
 Args    : a list of strings

_xref

 Title   : _xref
 Usage   : $self->_xref($value) 
 Function: an internal method to write db_xref elements
 Returns : nothing 
 Args    : a list of strings

_feature_span

 Title   : _feature_span
 Usage   : $self->_feature_span($name, $type, $loc)
 Function: an internal method to write a feature_span element
          (the actual feature with coordinates)
 Returns : nothing 
 Args    : a feature name and Bio::SeqFeatureI-compliant object

_seq_relationship

 Title   : _seq_relationship
 Usage   : $self->_seq_relationship($type, $loc)
 Function: an internal method to handle feature_span sequence relationships
 Returns : nothing
 Args    : feature type, a Bio::LocationI-compliant object,
           (optional) sequence name (defaults to the query seq)
           and (optional) alignment string

_element

 Title   : _element
 Usage   : $self->_element($name, $chars, $atts)
 Function: an internal method to generate 'generic' XML elements
 Example : 
 my $name = 'foo';
 my $content = 'bar';
 my $attributes = { baz => 1 }; 
 # print the element
 $self->_element($name, $content, $attributes);
 Returns : nothing 
 Args    : the element name and content plus a ref to an attribute hash

_span

 Title   : _span
 Usage   : $self->_span($loc)
 Function: an internal method to write the 'span' element
 Returns : nothing
 Args    : a Bio::LocationI-compliant object

_seq

 Title   : _seq
 Usage   : $self->_seq($seq, $dna) 
 Function: an internal method to print the 'sequence' element
 Returns : nothing
 Args    : and Bio::SeqI-compliant object and a reference to an attribute  hash

_find_name

 Title   : _find_name
 Usage   : my $name = $self->_find_name($feature)
 Function: an internal method to look for a gene name
 Returns : a string 
 Args    : a Bio::SeqFeatureI-compliant object

History