NAME
Bio::SeqFeature::Gene::GeneStructure - A feature representing an arbitrarily complex structure of a gene
SYNOPSIS
# See documentation of methods.
DESCRIPTION
A feature representing a gene structure. As of now, a gene structure really is only a collection of transcripts. See Bio::SeqFeature::Gene::TranscriptI (interface) and Bio::SeqFeature::Gene::Transcript (implementation) for the features of such objects.
FEEDBACK
Mailing Lists
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated.
[email protected] - General discussionhttp://bioperl.org/wiki/Mailing_lists - About the mailing lists
Support
Please direct usage questions or support issues to the mailing list:
rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.
Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
https://github.com/bioperl/bioperl-live/issues
AUTHOR - Hilmar Lapp
Email hlapp-at-gmx.net
APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
transcripts
Title : transcriptsUsage : @transcripts = $gene->transcripts();Function: Get the transcripts of this gene structure. Many gene structureswill have only one transcript.Returns : An array of Bio::SeqFeature::Gene::TranscriptI implementing objects.Args :
add_transcript
Title : add_transcript()Usage : $gene->add_transcript($transcript);Function: Add a transcript to this gene structure.Returns :Args : A Bio::SeqFeature::Gene::TranscriptI implementing object.
flush_transcripts
Title : flush_transcripts()Usage : $gene->flush_transcripts();Function: Remove all transcripts from this gene structure.Returns :Args :
add_transcript_as_features
Title : add_transcript_as_featuresUsage : $gene->add_transcript_as_features(@featurelist);Function: take a list of Bio::SeqFeatureI objects and turn them into aBio::SeqFeature::Gene::Transcript object. Add that transcript to the gene.Returns : nothingArgs : a list of Bio::SeqFeatureI compliant objects
promoters
Title : promotersUsage : @prom_sites = $gene->promoters();Function: Get the promoter features of this gene structure.This method basically merges the promoters returned by transcripts.Note that OO-modeling of regulatory elements is not stable yet.This means that this method might change or even disappear in afuture release. Be aware of this if you use it.Returns : An array of Bio::SeqFeatureI implementing objects.Args :
exons
Title : exons()Usage : @exons = $gene->exons();@inital_exons = $gene->exons('Initial');Function: Get all exon features or all exons of a specified type of this genestructure.Exon type is treated as a case-insensitive regular expression andoptional. For consistency, use only the following types:initial, internal, terminal, utr, utr5prime, and utr3prime.A special and virtual type is 'coding', which refers to all typesexcept utr.This method basically merges the exons returned by transcripts.Returns : An array of Bio::SeqFeature::Gene::ExonI implementing objects.Args : An optional string specifying the type of exon.
introns
Title : introns()Usage : @introns = $gene->introns();Function: Get all introns of this gene structure.Note that this class currently generates these features on-the-fly,that is, it simply treats all regions between exons as introns.It assumes that the exons in the transcripts do not overlap.This method basically merges the introns returned by transcripts.Returns : An array of Bio::SeqFeatureI implementing objects.Args :
poly_A_sites
Title : poly_A_sites()Usage : @polyAsites = $gene->poly_A_sites();Function: Get the poly-adenylation sites of this gene structure.This method basically merges the poly-adenylation sites returned bytranscripts.Returns : An array of Bio::SeqFeatureI implementing objects.Args :
utrs
Title : utrs()Usage : @utr_sites = $gene->utrs('3prime');@utr_sites = $gene->utrs('5prime');@utr_sites = $gene->utrs();Function: Get the features representing untranslated regions (UTR) of thisgene structure.You may provide an argument specifying the type of UTR. Currentlythe following types are recognized: 5prime 3prime for UTR on the5' and 3' end of the CDS, respectively.This method basically merges the UTRs returned by transcripts.Returns : An array of Bio::SeqFeature::Gene::ExonI implementing objectsrepresenting the UTR regions or sites.Args : Optionally, either 3prime, or 5prime for the the type of UTRfeature.
sub_SeqFeature
Title : sub_SeqFeatureUsage : @feats = $gene->sub_SeqFeature();Function: Returns an array of all subfeatures.This method is defined in Bio::SeqFeatureI. We override this hereto include the transcripts.Returns : An array Bio::SeqFeatureI implementing objects.Args : none
flush_sub_SeqFeature
Title : flush_sub_SeqFeatureUsage : $gene->flush_sub_SeqFeature();$gene->flush_sub_SeqFeature(1);Function: Removes all subfeatures.This method is overridden from Bio::SeqFeature::Generic to flushall additional subfeatures, i.e., transcripts, which isalmost certainly not what you want. To remove only features addedthrough $gene->add_sub_SeqFeature($feature) pass anyargument evaluating to TRUE.Example :Returns : noneArgs : Optionally, an argument evaluating to TRUE will suppress flushingof all gene structure-specific subfeatures (transcripts).