NAME
Bio::SearchIO::rnamotif - SearchIO-based RNAMotif parser
SYNOPSIS
# do not call this module directly. Use Bio::SearchIO.
DESCRIPTION
This is a highly experimental SearchIO-based parser for output from the rnamotif program (one of the programs in the RNAMotif suite). It currently parses only raw rnamotif output for RNAMotif versions 3.0 and above; older versions may work but will not be supported. rmfmt output will not be supported at this time.
FEEDBACK
Mailing Lists
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.
[email protected] - General discussionhttp://bioperl.org/wiki/Mailing_lists - About the mailing lists
Support
Please direct usage questions or support issues to the mailing list:
rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.
Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:
https://github.com/bioperl/bioperl-live/issues
AUTHOR - Chris Fields
Email cjfields-at-uiuc-dot-edu
APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
new
Title : newUsage : my $obj = Bio::SearchIO->new();Function: Builds a new Bio::SearchIO::rnamotif objectReturns : Bio::SearchIO::rnamotif parserArgs : -fh/-file => RNAMotif filename-format => 'rnamotif'-model => query model (or descriptor, in this case)-database => database name (default undef)-query_acc => query accession (default undef)-hsp_minscore => minimum HSP score cutoff-hsp_maxscore => maximum HSP score cutoff-symbols => hash ref of structure symbols to use(default symbols in %STRUCTURE_SYMBOLS hash)
next_result
Title : next_resultUsage : my $hit = $searchio->next_result;Function: Returns the next Result from a searchReturns : Bio::Search::Result::ResultI objectArgs : none
start_element
Title : start_elementUsage : $eventgenerator->start_elementFunction: Handles a start element eventReturns : noneArgs : hashref with at least 2 keys 'Data' and 'Name'
end_element
Title : start_elementUsage : $eventgenerator->end_elementFunction: Handles an end element eventReturns : noneArgs : hashref with at least 2 keys, 'Data' and 'Name'
element
Title : elementUsage : $eventhandler->element({'Name' => $name, 'Data' => $str});Function: Convenience method that calls start_element, characters, end_elementReturns : noneArgs : Hash ref with the keys 'Name' and 'Data'
element_hash
Title : elementUsage : $eventhandler->element_hash({'Hsp_hit-from' => $start,'Hsp_hit-to' => $end,'Hsp_score' => $lastscore});Function: Convenience method that takes multiple simple data elements andmaps to appropriate parametersReturns : noneArgs : Hash ref with the mapped key (in %MAPPING) and value
characters
Title : charactersUsage : $eventgenerator->characters($str)Function: Send a character eventsReturns : noneArgs : string
within_element
Title : within_elementUsage : if( $eventgenerator->within_element($element) ) {}Function: Test if we are within a particular elementThis is different than 'in' because within can be testedfor a whole block.Returns : booleanArgs : string element name
in_element
Title : in_elementUsage : if( $eventgenerator->in_element($element) ) {}Function: Test if we are in a particular elementThis is different than 'within' because 'in' onlytests its immediate parent.Returns : booleanArgs : string element name
start_document
Title : start_documentUsage : $eventgenerator->start_documentFunction: Handle a start document eventReturns : noneArgs : none
end_document
Title : end_documentUsage : $eventgenerator->end_documentFunction: Handles an end document eventReturns : Bio::Search::Result::ResultI objectArgs : none
result_count
Title : result_countUsage : my $count = $searchio->result_countFunction: Returns the number of results we have processedReturns : integerArgs : none
descriptor
Title : descriptorUsage : my $descr = $parser->descriptor();Function: Get/Set descriptor name. Some versions of RNAMotif do not add thedescriptor name to the output. This also overrides any name foundwhile parsing.Returns : String (name of model)Args : [optional] String (name of model)
model
Title : modelUsage : my $model = $parser->model();Function: Get/Set model; Infernal currently does not outputthe model name (Rfam ID)Returns : String (name of model)Args : [optional] String (name of model)Note : this is a synonym for descriptor()
database
Title : databaseUsage : my $database = $parser->database();Function: Get/Set database; Infernal currently does not outputthe database nameReturns : String (database name)Args : [optional] String (database name)
query_accession
Title : query_accessionUsage : my $acc = $parser->query_accession();Function: Get/Set query (model) accession; RNAMotif currently does not outputthe accession numberReturns : String (accession)Args : [optional] String (accession)
algorithm_version
Title : algorithm_versionUsage : my $ver = $parser->algorithm_version();Function: Get/Set algorithm version (not defined in RNAMotif output)Returns : String (accession)Args : [optional] String (accession)
hsp_minscore
Title : hsp_minscoreUsage : my $cutoff = $parser->hsp_minscore();Function: Get/Set min score cutoff (for generating Hits/HSPs).Returns : score (number)Args : [optional] score (number)Note : Cannot be set along with hsp_maxscore()
hsp_maxscore
Title : hsp_maxscoreUsage : my $cutoff = $parser->hsp_maxscore();Function: Get/Set max score cutoff (for generating Hits/HSPs).Returns : score (number)Args : [optional] score (number)Note : Cannot be set along with hsp_minscore()
structure_symbols
Title : structure_symbolsUsage : my $hashref = $parser->structure_symbols();Function: Get/Set RNA structure symbolsReturns : Hash ref of delimiters (5' stem, 3' stem, single-strand, etc): default = < (5-prime)> (3-prime). (single-strand)? (unknown)Args : Hash ref of substitute delimiters, using above keys.
_motif2meta
Title : _motif2metaUsage : my ($rna, $meta) = $parser->_motif2meta($str, $descr);Function: Creates meta string from sequence and descriptorReturns : array of sequence, meta stringsArgs : Array of string data and descriptor dataNote: This is currently a quick and simple way of making simpleRNA structures (stem-loops, helices, etc) from RNAMotif descriptordata in the output file. It does not currently work with pseudoknots,triplets, G-quartets, or other more complex RNA structural motifs.