---
name: "Bio::PrimerDesigner::epcr"
section: "3pm"
platform: "linux"
package: "libbio-primerdesigner-perl"
version: "0.07-8"
release: "Debian 13 (Trixie)"
platforms: ["linux"]
url: "https://new.carta.tech/Bio::PrimerDesigner::epcr"
---

# Bio::PrimerDesigner::epcr(3pm)

A class for accessing the epcr binary

## NAME

Bio::PrimerDesigner::epcr - A class for accessing the epcr binary

## SYNOPSIS

```
  use Bio::PrimerDesigner::epcr;
```

## DESCRIPTION

A low-level interface to the e-PCR binary.  Uses supplied PCR primers, DNA sequence and stringency parameters to predict both expected  and unexpected PCR products.

## METHODS

### run

Sets up the e-PCR request for a single primer combination and returns  an Bio::PrimerDesigner::Result object

If the permute flag is true, all three possible primer combinations  will be tested (ie: forward + reverse, forward + forward, reverse + reverse)

### request

Assembles the e-PCR config file and command-line arguments and send the e-PCR request to the local e-PCR binary or remote server.

### verify

Check to make that the e-PCR binary is installed and functioning properly.  Since e-PCR returns nothing if no PCR product is found in the sequence, we have to be able to distinguish between a valid, undefined output from a functioning e-PCR and an undefined output for some other reason.  verify uses sham e-PCR data that is known  to produce a PCR product.

### binary\_name

Defines the binary's name on the system.

### list\_aliases

There are no aliases to list for epcr.

### list\_params

Returns a list of e-PCR configuration options.  Required e-PCR input is a sequence string or file and the left and right primers.  Default values will be used for the remaining options if none are supplied.

## AUTHOR

Copyright (C) 2003-2009 Sheldon McKay \<mckays@cshl.edu\>, Ken Youens-Clark \<kclark@cpan.org\>.

## LICENSE

This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; version 3 or any later version.

This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the GNU General Public License for more details.

You should have received a copy of the GNU General Public License along with this program; if not, write to the Free Software Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301 USA.

## SEE ALSO

Bio::PrimerDesigner::primer3.