NAME
Bio::LiveSeq::Transcript - Transcript class for LiveSeq
SYNOPSIS
# documentation needed
DESCRIPTION
This stores information about coding sequences (CDS). The implementation is that a Transcript object accesses a collection of Exon objects, inferring from them the nucleotide structure and sequence.
AUTHOR - Joseph A.L. Insana
Email: [email protected], [email protected]
APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
new
Title : newUsage : $transcript = Bio::LiveSeq::Transcript->new(-exons => \@obj_refs);Function: generates a new Bio::LiveSeq::TranscriptReturns : reference to a new object of class TranscriptErrorcode -1Args : reference to an array of Exon object references
all_Exons
Title : all_ExonsUsage : $transcript_obj->all_Exons()Function: returns references to all Exon objects the Transcript is composed ofExample : foreach $exon ($transcript->all_Exons()) { do_something }Returns : array of object referencesArgs : none
downstream_seq
Title : downstream_seqUsage : $transcript_obj->downstream_seq(): $transcript_obj->downstream_seq(64)Function: returns a string of nucleotides downstream of the end of theCDS. If there is some information of the real mRNA, from features inan attached Gene object, it will return up to those boundaries.Otherwise it will return 1000 nucleotides.If an argument is given it will override the default 1000 numberand return instead /that/ requested number of nucleotides.But if a Gene object is attached, this argument will be ignored.Returns : stringArgs : an optional integer number of nucleotides to be returned instead ofthe default if no gene attached
upstream_seq
Title : upstream_seqUsage : $transcript_obj->upstream_seq(): $transcript_obj->upstream_seq(64)Function: just like downstream_seq but returns nucleotides before the ATGNote : the default, if no Gene information present and no nucleotidesnumber given, is to return up to 400 nucleotides.
get_Translation
Title : validUsage : $translation = $obj->get_Translation()Function: retrieves the reference to the object of class Translation (if any)attached to a LiveSeq objectReturns : object referenceArgs : none
translation_table
Title : translation_tableUsage : $name = $obj->translation_table;: $name = $obj->translation_table(11);Function: Returns or sets the translation_table used for translating thetranscript.If it has never been set, it will return undef.Returns : an integer
frame
Title : frameUsage : $frame = $transcript->frame($label);Function: Returns the frame of a particular nucleotide.Frame can be 0 1 or 2 and means the position in the codon tripletof the particulat nucleotide. 0 is the first codon_position.Codon_position (1 2 3) is simply frame+1.If the label asked for is not inside the Transcript, -1 will bereturned.Args : a labelReturns : 0 1 or 2Errorcode -1