NAME
Bio::Factory::SequenceProcessorI - Interface for chained sequence
processing algorithms
SYNOPSIS
use Bio::SeqIO;use MySeqProcessor; # is-a Bio::Factory::SequenceProcessorI# obtain your source stream, e.g., an EMBL filemy $seqin = Bio::SeqIO->new(-fh => \*STDIN, -format => 'embl');# create your processor (it must implement this interface)my $seqalgo = MySeqProcessor->new();# chain together$seqalgo->source_stream($seqin);# you could create more processors and chain them one after another# ...# finally, the last link in the chain is your SeqIO streammy $seqpipe = $seqalgo;# once you've established the pipeline, proceed as if you had a# single SeqIO streamwhile(my $seq = $seqpipe->next_seq()) {# ... do something ...}
DESCRIPTION
This defines an interface that allows seamless chaining of sequence processing algorithms encapsulated in modules while retaining the overall Bio::SeqIO interface at the end of the pipeline.
This is especially useful if you want an easily configurable processing pipeline of re-usable algorithms as building blocks instead of (hard-)coding the whole algorithm in a single script.
There are literally no restrictions as to what an individual module can do with a sequence object it obtains from the source stream before it makes it available through its own next_seq() method. It can manipulate the sequence object, but otherwise keep it intact, but it can also create any number of new sequence objects from it, or it can discard some, or any combination thereof. The only requirement is that its next_seq() method return Bio::PrimarySeqI compliant objects. In order to play nice, if a processor creates new objects it should try to use the same sequence factory that the source stream uses, but this is not strongly mandated.
FEEDBACK
Mailing Lists
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.
[email protected] - General discussionhttp://bioperl.org/wiki/Mailing_lists - About the mailing lists
Support
Please direct usage questions or support issues to the mailing list:
rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.
Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:
https://github.com/bioperl/bioperl-live/issues
AUTHOR - Hilmar Lapp
Email hlapp at gmx.net
APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
source_stream
Title : source_streamUsage : $obj->source_stream($newval)Function: Get/set the source sequence stream for this sequenceprocessor.An implementation is not required to allow set, but willusually do so.Example :Returns : A Bio::Factory::SequenceStreamI compliant objectArgs : on set, new value (a Bio::Factory::SequenceStreamI compliantobject)
Bio::Factory::SequenceStreamI methods
The requirement to implement these methods is inherited fromL<Bio::Factory::SequenceStreamI>. An implementation may notnecessarily have to implement all methods in a meaningful way. Whichmethods will be necessary very much depends on the context in whichan implementation of this interface is used. E.g., if it is only usedfor post-processing sequences read from a SeqIO stream, write_seq()will not be used and hence does not need to be implemented in ameaningful way (it may in fact even throw an exception).Also, since an implementor will already receive built objects from asequence stream, sequence_factory() may or may not be relevant,depending on whether the processing method does or does not involvecreating new objects.
next_seq
Title : next_seqUsage : $seq = stream->next_seqFunction: Reads the next sequence object from the stream and returns it.In the case of a non-recoverable situation an exceptionwill be thrown. Do not assume that you can resume parsingthe same stream after catching the exception. Note that youcan always turn recoverable errors into exceptions bycalling $stream->verbose(2).Returns : a Bio::Seq sequence objectArgs : none
See Bio::Root::RootI
write_seq
Title : write_seqUsage : $stream->write_seq($seq)Function: writes the $seq object into the streamReturns : 1 for success and 0 for errorArgs : Bio::Seq object
sequence_factory
Title : sequence_factoryUsage : $seqio->sequence_factory($seqfactory)Function: Get the Bio::Factory::SequenceFactoryIReturns : Bio::Factory::SequenceFactoryIArgs : none