Barcode_split(1)

optional arguments:

-h, --help

show this help message and exit

--version

show program's version number and exit

--summary_file [SUMMARY_FILE ...], -f [SUMMARY_FILE ...]

Path to a sequencing_summary generated by Albacore 1.0.0 + (read_fast5_basecaller.py) / Guppy 2.1.3+ (guppy_basecaller). One can also pass multiple space separated file paths or a UNIX style regex matching multiple files

--barcode_file [BARCODE_FILE ...], -b [BARCODE_FILE ...]

Path to the barcode_file generated by Guppy 2.1.3+ (guppy_barcoder) or Deepbinner 0.2.0+. One can also pass multiple space separated file paths or a UNIX style regex matching multiple files

--output_dir OUTPUT_DIR, -o OUTPUT_DIR

Folder where to output split barcode data (default: current dir

--output_unclassified, -u

If given, unclassified barcodes are also written in a file. By default they are skiped

--min_barcode_percent MIN_BARCODE_PERCENT, -p MIN_BARCODE_PERCENT

Minimal percent of total reads to retain barcode label. If below, the barcode value is set as `unclassified` (default: 0.1)

-v, --verbose

Increase verbosity

-q, --quiet

Reduce verbosity